TLX1 antibody (AA 300-330, AA 306-318)

Catalog No. ABIN297247

This Rabbit Polyclonal antibody specifically detects TLX1 in WB, IHC (p) and IHC. It exhibits reactivity toward Mouse, Human, Rat, Cow, Dog, Rabbit, Bat, Monkey, Chicken, Horse and Hamster.

Quick Overview for TLX1 antibody (AA 300-330, AA 306-318) (ABIN297247)

Target: TLX1 (T-Cell Leukemia Homeobox 1 (TLX1))

Reactivity: Mouse, Human, Rat, Cow, Dog, Rabbit, Bat, Monkey, Chicken, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-TLX1 Antibody

Binding Specificity: AA 300-330, AA 306-318

Brand: IHC-plus™

Specificity: A portion of amino acids 300-330 of human HOX11 protein was used as the immunogen.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Turkey, Chicken (100%) Opossum, Platypus, Xenopus (85%).

Purification: Protein G purified

Immunogen: A peptide sequence corresponding to acids amino acids 306-318 (LQNLQPWSDDSTK) of human HOX11was used as the immunogen, GenBank no CAA08834.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Turkey, Chicken (100%), Opossum, Platypus, Xenopus (85%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TLX1

Target: TLX1 (T-Cell Leukemia Homeobox 1 (TLX1))

Alternative Name: TLX1 / HOX11

Background: Name/Gene ID: TLX1

Synonyms: TLX1, Homeobox protein Hox-11, HOX11, T-cell leukemia homeobox 1, Proto-oncogene TCL-3, TCL3, T-cell leukemia, homeobox 1

Gene ID: 3195

UniProt: P31314