Lipin 1 antibody (AA 448-574)

Catalog No. ABIN297253

This Goat Polyclonal antibody specifically detects Lipin 1 in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for Lipin 1 antibody (AA 448-574) (ABIN297253)

Target: Lipin 1 (LPIN1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Lipin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Lipin 1 Antibody

Binding Specificity: AA 448-574

Specificity: Selected for its ability to recognize human Lipin 1 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 2 % cross-reactivity with rhLipin2 and rhLipin3.

Purification: Affinity purified

Immunogen: E. coli-derived rhLipin 1 (aa448-574).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human Lipin 1 on human MSC-derived adipocytes. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 2 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 0.1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Lipin 1

Target: Lipin 1 (LPIN1)

Alternative Name: LPIN1 / Lipin 1

Background: Name/Gene ID: LPIN1

Synonyms: LPIN1, Lipin-1, Lipin 1, PAP1, KIAA0188

Gene ID: 23175

UniProt: Q14693

Pathways: Monocarboxylic Acid Catabolic Process