SET/TAF-I antibody (AA 66-81)

Catalog No. ABIN297641

The Rabbit Polyclonal anti-SET/TAF-I antibody is suitable to detect SET/TAF-I in samples from Human, Mouse, Rat, Chicken, Xenopus laevis, Zebrafish (Danio rerio), Cow, Horse, Monkey, Pig and Hamster. It has been validated for WB, IHC and IHC (p).

Quick Overview for SET/TAF-I antibody (AA 66-81) (ABIN297641)

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Reactivity: Human, Mouse, Rat, Chicken, Xenopus laevis, Zebrafish (Danio rerio), Cow, Horse, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SET/TAF-I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SET/TAF-I Antibody

Binding Specificity: AA 66-81

Specificity: An approx. 37 kD band is observed.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%) Gorilla, Stickleback (94%) Dog (88%).

Purification: Protein G purified

Immunogen: Synthetic peptides of amino acids 66-81 (PFFQKRSELIAKIPNF) and 135-151 (ENKVLSKEFHLNESGDP) of human INHAT-1. Sequence homology is 100% in mouse and rat. Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%), Gorilla, Stickleback (94%), Dog (88%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 mg/mL BSA. sodium azide is toxic.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SET/TAF-I

Target: SET/TAF-I (SET) (SET Nuclear Oncogene (SET))

Alternative Name: SET / TAF-I

Background: Name/Gene ID: SET

Synonyms: SET, 2PP2A, HLA-DR-associated protein II, I-2PP2A, TAF-IBETA, SET nuclear oncogene, TAF-I, Template-activating factor I, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET

Gene ID: 6418

UniProt: Q01105

Pathways: Apoptosis