SORBS1 antibody (AA 1282-1294)

Catalog No. ABIN297694

This Goat Polyclonal antibody specifically detects SORBS1 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Hamster, Mouse, Rat, Dog, Horse, Monkey and Bat.

Quick Overview for SORBS1 antibody (AA 1282-1294) (ABIN297694)

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Reactivity: Human, Hamster, Mouse, Rat, Dog, Horse, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This SORBS1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SORBS1 Antibody

Binding Specificity: AA 1282-1294

Specificity: Human SORBS1. This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Hamster, Elephant, Panda, Horse, Opossum, Platypus (100%) Pig, Turkey, Chicken (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GTFPGNYVKPLYL from the C-terminus of human SORBS1 (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Hamster, Elephant, Panda, Horse, Opossum, Platypus (100%), Pig, Turkey, Chicken (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SORBS1

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Alternative Name: SORBS1 / Ponsin

Background: Name/Gene ID: SORBS1

Synonyms: SORBS1, C-Cbl associated protein, C-Cbl-associated protein, CAP, FLAF2, KIAA0894, KIAA1296, R85FL, SH3 domain protein 5, SH3-domain protein 5 (ponsin), SH3P12, SORB1, Fas-ligand associated factor 2, Ponsin, SH3D5

Gene ID: 10580

NCBI Accession: NP_006425, NP_056200, NP_001030126, NP_001030127, NP_001030128, NP_079267, NP_001030129

UniProt: Q9BX66

Pathways: Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process