SOX15 antibody

Catalog No. ABIN297700

This Sheep Polyclonal antibody specifically detects SOX15 in WB, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for SOX15 antibody (ABIN297700)

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This SOX15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-SOX15 Antibody

Specificity: Selected for its ability to recognize human SOX15 in the applications listed.

Purification: Affinity purified

Immunogen: E. coli-derived rhSOX15.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1 % cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for SOX15

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Alternative Name: SOX15

Background: Name/Gene ID: SOX15

Synonyms: SOX15, Protein SOX-15, Protein SOX-20, SOX26, SOX20, Protein SOX-12, SOX27

Gene ID: 6665

UniProt: O60248