GRK5 antibody (Internal Region)

Catalog No. ABIN297746

The Rabbit Polyclonal anti-GRK5 antibody has been validated for IHC and IHC (p). It is suitable to detect GRK5 in samples from Human, Mouse, Rat, Cow, Horse, Chicken, Monkey, Pig and Bat.

Quick Overview for GRK5 antibody (Internal Region) (ABIN297746)

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Reactivity: Human, Mouse, Rat, Cow, Horse, Chicken, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRK5 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Human GRK5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Bovine, Panda, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%) Orangutan, Dog, Elephant, Xenopus (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human GRK5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Bovine, Panda, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%), Orangutan, Dog, Elephant, Xenopus (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 7 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GRK5

Target: GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))

Alternative Name: GRK5

Background: Name/Gene ID: GRK5
Subfamily: GPRK
Family: Protein Kinase

Synonyms: GRK5, FP2025, GPRK5

Gene ID: 2869

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling