IDS antibody (AA 26-550)

Catalog No. ABIN298150

This Goat Polyclonal antibody specifically detects IDS in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for IDS antibody (AA 26-550) (ABIN298150)

Target: IDS (Iduronate 2-Sulfatase (IDS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IDS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-IDS Antibody

Binding Specificity: AA 26-550

Specificity: Selected for its ability to recognize human IDS in direct ELISAs and Western Blots. In these formats, this antibody shows ~50 % cross-reactivity with rmIDS.

Purification: Affinity purified

Immunogen: NS0-derived rhIDS (aa26-550).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human IDS. The detection limit for rhIDS is approximately 25 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhIDS from conditioned media of transfected NS0 cells. The recovered rhIDS can be detected by Western Blot analysis. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human IDS. The detection limit for rhIDS is approximately 0.3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 500 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Target Details for IDS

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: IDS

Background: Name/Gene ID: IDS

Synonyms: IDS, Idursulfase, SIDS, Iduronate 2-sulfatase, MPS2

Gene ID: 3423

UniProt: P22304

Pathways: Glycosaminoglycan Metabolic Process