CD5 antibody (FITC)

Catalog No. ABIN298316

The Rat Monoclonal anti-CD5 antibody has been validated for FACS, IHC, IP and IHC (fro). It is suitable to detect CD5 in samples from Dog.

Quick Overview for CD5 antibody (FITC) (ABIN298316)

Target: CD5

Reactivity: Dog

Host: Rat

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YKIX322-3

Product Details anti-CD5 Antibody (FITC)

Specificity: Recognizes canine CD5, a 67 kD cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels. Was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop. In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression. Serves as a useful marker for the discrimination of canine leukemias of differing origins.

Purification: Affinity purified

Immunogen: Concanavalin A activated canine peripheral blood cells.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:10), IHC, IHC-Fr, IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Method sheets are available on request.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: Name/Gene ID: CD5

Synonyms: CD5, CD5 antigen, CD5 antigen (p56-62), CD5 Molecule, Lymphocyte antigen T1/Leu-1, LEU1, T1

Gene ID: 921