CMA1 antibody (AA 20-247)

Catalog No. ABIN300998

The Sheep Polyclonal anti-CMA1 antibody has been validated for WB, ELISA and IP. It is suitable to detect CMA1 in samples from Human.

Quick Overview for CMA1 antibody (AA 20-247) (ABIN300998)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CMA1 Antibody

Binding Specificity: AA 20-247

Specificity: Recognizes human Chymase. Cross-reactivity: Approximately 10 % with r  mMCPT1 and <1 % with r  mMCPT6.

Purification: Affinity purified

Immunogen: NS0-derived recombinant human Chymase (aa20-247) Accession # P23946.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Western Blot: 0.1-0.2 μg/mL. Detection limit is ~5 ng/lane under non-reducing or reducing conditions. ELISA: 0.5-1 μg/mL. Detection limit is ~1.5 ng/well. Immunoprecipitation: Immunoprecipitate rhChymase. from conditioned media of transfected NS0 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 μL of Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C for short-term only. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: CMA1 / MCT1 / Chymase

Background: Name/Gene ID: CMA1
Subfamily: Serine S1
Family: Protease

Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM

Gene ID: 1215

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis