Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 1-350) antibody

Catalog No. ABIN3015830

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Human.

Quick Overview for Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 1-350) antibody (ABIN3015830)

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 1-350

Sequence: MANSMNGRNP GGRGGNPRKG RILGIIDAIQ DAVGPPKQAA ADRRTVEKTW KLMDKVVRLC QNPKLQLKNS PPYILDILPD TYQHLRLILS KYDDNQKLAQ LSENEYFKIY IDSLMKKSKR AIRLFKEGKE RMYEEQSQDR RNLTKLSLIF SHMLAEIKAI FPNGQFQGDN FRITKADAAE FWRKFFGDKT IVPWKVFRQC LHEVHQISSG LEAMALKSTI DLTCNDYISV FEFDIFTRLF QPWGSILRNW NFLAVTHPGY MAFLTYDEVK ARLQKYSTKP GSYIFRLSCT RLGQWAIGYV TGDGNILQTI PHNKPLFQAL IDGSREGFYL YPDGRSYNPD LTGLCEPTPH

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CBLB (NP_733762.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Alternative Name: CBLB

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor, BCR (B-cell receptor and FCER1 (high affinity immunoglobulin epsilon receptor signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA signaling pathway via ubiquitination and internalization of PDGFRA (By similarity.,CBLB,Cbl-b,Nbla00127,RNF56,Cancer,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Endocrine & Metabolism,Insulin Receptor Signaling Pathway,Immunology & Inflammation,B Cell Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,CBLB

Molecular Weight: 86 kDa/90 kDa/109 kDa

Gene ID: 868

UniProt: Q13191