Histone H4 antibody (2meLys20)

Catalog No. ABIN3016048

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC, IF, IP, ChIP and ChIP-seq. It exhibits reactivity toward Human.

Quick Overview for Histone H4 antibody (2meLys20) (ABIN3016048)

Target: Histone H4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone H4 Antibody

Binding Specificity: 2meLys20

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Methylated Antibodies

Purification: Affinity purification

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Histone H4

Target: Histone H4

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the centromeric copy.,FO108,H4,H4/n,H4F2,H4FN,HIST2H4,Histone H4,HIST1H4A,HIST2H4A,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H4

Molecular Weight: 11 kDa

Gene ID: 8370

UniProt: P62805