FZD7 antibody (AA 33-185) (Biotin)

Catalog No. ABIN301685

The Goat Polyclonal anti-FZD7 antibody has been validated for WB. It is suitable to detect FZD7 in samples from Mouse.

Quick Overview for FZD7 antibody (AA 33-185) (Biotin) (ABIN301685)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD7 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Product Details anti-FZD7 Antibody (Biotin)

Binding Specificity: AA 33-185

Specificity: Recognizes mouse Frizzled-7 extracellular Cysteine-Rich Domain.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-7 (aa33-185).

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: Use at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-7. The detection limit for rmFrizzled-7 is ~1 ng/lane under non-reducing and reducing. conditions. In this format, this antibody shows ~5 % cross-reactivity with rmFrizzled-2 and less than 2 % cross-reactivity with rmFrizzled-3, rmFrizzled-4, rhFrizzled-5, rmFrizzled-6, rmFrizzled-8 and rmFrizzled-9. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, BSA. Labeled, Biotin.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7 / Frizzled 7

Background: Name/Gene ID: FZD7
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD7, Frizzled family receptor 7, Frizzled homolog 7, Frizzled 7, Frizzled-7, FzE3, Fz-7, HFz7

Gene ID: 8324

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance