LIMK2 antibody (pThr505)

Catalog No. ABIN3020313

This Rabbit Polyclonal antibody specifically detects LIMK2 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for LIMK2 antibody (pThr505) (ABIN3020313)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LIMK2 Antibody

Binding Specificity: pThr505

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Immunogen: A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:100,IF,1:100 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIMK2

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.,LIMK2,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Kinase,Serine/threonine kinases,Cell Biology & Developmental Biology,Cytoskeleton,Microfilaments,TGF-b-Smad Signaling Pathway,Protein phosphorylation,LIMK2

Molecular Weight: 69 kDa/72 kDa/77 kDa

Gene ID: 3985

UniProt: P53671