DYKDDDDK Tag antibody

Catalog No. ABIN3020559

This Mouse Monoclonal antibody specifically detects DYKDDDDK Tag in WB, IP and IF. It has been mentioned in 11+ publications

Quick Overview for DYKDDDDK Tag antibody (ABIN3020559)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DYKDDDDK Tag Antibody

Sequence: DYKDDDDK

Purification: Affinity purification

Immunogen: Fusion protein of human DYKDDDDK-Tag

Isotype: IgG

Application Details

Application Notes: WB: 1:5000, IF: 1:2000, IP: 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C / -80 °C

References for anti-DYKDDDDK Tag antibody (ABIN3020559)

Liang, Li, Jin, Xu, Pi, Liu, Zhu, Zhang, Luan, Gong, Zhang: "Pharmacological effect of human melanocortin-2 receptor accessory protein 2 variants on hypothalamic melanocortin receptors." in: Endocrine, Vol. 61, Issue 1, pp. 94-104, (2019) (PubMed).

Liu, Xu, Zhu, Zhang, Chen, Zhou, Chen, Lin: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).

Wang, Yao, Wang, Ma, Tong, Wang, Bao, Jiang, Yang, Zhang, Xu, Wang, Zhang, Wang: "Overexpression of VpEIFP1, a novel F-box/Kelch-repeat protein from wild Chinese Vitis pseudoreticulata, confers higher tolerance to powdery mildew by inducing thioredoxin z proteolysis." in: Plant science : an international journal of experimental plant biology, Vol. 263, pp. 142-155, (2018) (PubMed).

He, Wang, Higgins, Yu, Zong, Lu, Zhang, Liang: "MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice." in: The Plant cell, Vol. 28, Issue 8, pp. 1879-93, (2017) (PubMed).

Jing, Zhou, Fang, Ding, Wang, Ke, Chen, Xiao: "DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection." in: Frontiers in immunology, Vol. 8, pp. 1365, (2017) (PubMed).

Liu, Xin, Wang, Wang, Wang, Zhang, Yang, Zhang, Zhang, Wang, Xu, Duan, Xia: "Estrogen receptors in granulosa cells govern meiotic resumption of pre-ovulatory oocytes in mammals." in: Cell death & disease, Vol. 8, Issue 3, pp. e2662, (2017) (PubMed).

Ye, Chen, Li, Zhao, He, Zohaib, Song, Deng, Zhang, Chen, Cao: "Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

Jing, Fang, Ding, Wang, Hao, Gao, Ke, Chen, Xiao: "Porcine Reproductive and Respiratory Syndrome Virus nsp1α Inhibits NF-κB Activation by Targeting the Linear Ubiquitin Chain Assembly Complex." in: Journal of virology, Vol. 91, Issue 3, (2017) (PubMed).

Chen, Ye, Ashraf, Li, Wei, Wan, Zohaib, Song, Chen, Cao: "MicroRNA-33a-5p Modulates Japanese Encephalitis Virus Replication by Targeting Eukaryotic Translation Elongation Factor 1A1." in: Journal of virology, Vol. 90, Issue 7, pp. 3722-34, (2016) (PubMed).

Fan, Zhang, Qian, Qian, Wu, Chen, Li: "Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism." in: Antiviral research, Vol. 127, pp. 32-40, (2016) (PubMed).

Li, Liu, Yang, Fu, Zhao, Shen, Miao, Rayner, Chavanas, Zhu, Britt, Tang, McVoy, Luo: "Human Cytomegalovirus Infection Dysregulates the Localization and Stability of NICD1 and Jag1 in Neural Progenitor Cells." in: Journal of virology, Vol. 89, Issue 13, pp. 6792-804, (2015) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: FLAG tag

Target Type: Tag

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(DYKDDDDK) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DYKDDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DYKDDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DYKDDDDK peptide is more likely to be accessible to antibodies. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against3xDYKDDDDK DYKDDDDK sequence and recognizes the (3x)DYKDDDDK peptide and detects DYKDDDDK-Tagged proteins.