Mouse anti-Human IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN302063

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (HRP) (ABIN302063)

Target: IgG

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Clone: EM-07

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Purpose: Anti-Hu IgG (Fc) HRP

Specificity: The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

Cross-Reactivity (Details): Human, Other not determined

Purification: Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

Immunogen: Fusion protein of human IgG Fc fragment.

Application Details

Application Notes: Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody

Background: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

Molecular Weight: 150 kDa