DDDDK Tag antibody

Catalog No. ABIN302065

This Mouse Monoclonal antibody specifically detects DDDDK Tag in WB and ICC. It has been mentioned in 2+ publications

Quick Overview for DDDDK Tag antibody (ABIN302065)

Target: DDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: F-tag-01

Product Details anti-DDDDK Tag Antibody

Purpose: Anti-DDDDK tag Purified

Sequence: DYKDDDDK

Cross-Reactivity (Details): Recognizes fusion proteins in all species

Characteristics: The mouse monoclonal antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide: DYKDDDDK conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-DDDDK Tag antibody (ABIN302065)

Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, (2009) (PubMed).

Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

Target Details for DDDDK Tag

Target: DDDDK Tag

Target Type: Tag

Gene ID: 35