CD69 antibody

Catalog No. ABIN302090

The Mouse Monoclonal anti-CD69 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD69 in samples from Human. There are 3+ publications available.

Quick Overview for CD69 antibody (ABIN302090)

Target: CD69

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD69 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: FN50

Product Details anti-CD69 Antibody

Purpose: Anti-Hu CD69 Purified

Specificity: The mouse monoclonal antibody FN50 recognizes an extracellular epitope of CD69, an lymphocyte early activation marker.

Cross-Reactivity (Details): Human, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Anti-µ-stimulated human B lymphocytes

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD69 antibody (ABIN302090)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, (2007) (PubMed).

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background: CD69 Molecule,CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.,EA1, AIM, MLR-3, CLEC2C, GP32/28, BL-AC/P26

Gene ID: 969

UniProt: Q07108