Teratocarcinoma-Derived Growth Factor 1 (TDGF1) antibody

Details for Product No. ABIN3021342
Antigen
Reactivity
Mouse (Murine), Rat (Rattus), Human
159
50
42
Host
Rabbit
117
40
7
2
Clonality
Polyclonal
Conjugate
Un-conjugated
15
14
14
6
5
5
5
5
5
5
5
5
4
4
4
4
4
Application
Immunohistochemistry (IHC), Western Blotting (WB)
72
65
52
52
16
5
5
4
2
2
1
Options
Immunogen Recombinant protein of human TDGF1
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name TDGF1 (TDGF1 Antibody Abstract)
Background Cripto, also known as teratocarcinoma derived growth factor 1 (TDGF-1), belongs to the EGF-CFC family of proteins. Members of this family are characterized by an N-terminal signal peptide, a conserved cysteine rich domain (CFC motif), and a short hydrophobic carboxy-terminal tail that contains GPI cleavage and attachment sites. The GPI moiety anchors Cripto and family members to the extracellular plasma membrane. An O-linked fucosylation site within the EGF-like motif is required for Cripto and related family members to perform their function as co-receptors for TGF-beta-related ligands such as Nodal and Vg1/GDF1. Soluble forms of Cripto can be produced - these contain intact EGF and CFC domains, and are thought to have paracrine activities, as opposed to the autocrine activity of Cripto functioning as a coreceptor. Understanding of this paracrine activity is not complete, but it is proposed that Cripto may act as co-ligand for Nodal.Cripto is an important modulator of embryogenesis and oncogenesis. It is highly expressed in early embryos, and in embryonic stem (ES) cells where it is involved in cardiomyocytic differentiation and acts as a negative regulator of neurogenesis. Transient activation of Cripto is essential for the capacity of stem cell self-renewal and pluripotency in ES cells, and in some adult derived stem cells. Signaling through Cripto can also stimulate other activities that promote tumorigenesis such as stimulation of proliferation, cell motility, invasion, angiogenesis and epithelial-mesenchymal transition (EMT). Cripto is highly expressed in a broad range of tumors, where it acts as a potent oncogene.
Synonyms: TDGF1, CR, CRGF, CRIPTO, Cripto-1
Gene ID 6997
UniProt P13385
Pathways EGFR Signaling Pathway
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C
Supplier Images
Image no. 1 for anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) antibody (ABIN3021342) Western blot analysis of NCCIT cell lysate using TDGF1 antibody.