EGLN3 antibody (Internal Region)

Catalog No. ABIN302149

This Goat Polyclonal antibody specifically detects EGLN3 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for EGLN3 antibody (Internal Region) (ABIN302149)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-EGLN3 Antibody

Binding Specificity: Internal Region

Purpose: EGLN3

Sequence: RLGKYYVKER SK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-RLGKYYVKERSK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 30 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 27.7 kDa according to NP_071356.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3

Background: EGLN3 , egl nine homolog 3 (C. elegans) , FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3, EGL nine (C.elegans) homolog 3, egl nine homolog 3, egl nine-like protein 3 isoform

Gene ID: 112399, 112407, 54702

NCBI Accession: NP_071356

Pathways: Positive Regulation of Endopeptidase Activity