ABCC11 antibody (Internal Region)

Catalog No. ABIN302164

This Goat Polyclonal antibody specifically detects ABCC11 in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for ABCC11 antibody (Internal Region) (ABIN302164)

Target: ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ABCC11 Antibody

Binding Specificity: Internal Region

Purpose: MRP8 / ABCC11

Sequence: KVVEFDRPEV LRK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KVVEFDRPEVLRK, from the internal region (near C Terminus) of the protein sequence according to NP_115972.2, NP_660187.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 125 kDa band observed in Human Testis lysates and in preliminary testing of Breast lysate (calculated MW of 150 kDa according to NP_660187.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentrat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ABCC11

Target: ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))

Alternative Name: ABCC11

Background: ABCC11, ATP-binding cassette transporter MRP8, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8, WW, ATP-binding cassette protein C11, ATP-binding cassette, sub-family C, member 11, multi-resistance protein 8

Gene ID: 85320

NCBI Accession: NP_115972, NP_660187