Apolipoprotein F antibody (C-Term)

Catalog No. ABIN302173

This Goat Polyclonal antibody specifically detects Apolipoprotein F in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Apolipoprotein F antibody (C-Term) (ABIN302173)

Target: Apolipoprotein F (APOF)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Apolipoprotein F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Apolipoprotein F Antibody

Binding Specificity: C-Term

Purpose: Apolipoprotein F

Sequence: KSYDLDPGAG SLE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KSYDLDPGAGSLE, from the C Terminus of the protein sequence according to NP_001629.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Approx. 35 kDa band observed in Human Liver lysates (calculated MW of 35.4 kDa according to NP_001629.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Apolipoprotein F

Target: Apolipoprotein F (APOF)

Alternative Name: APOF

Background: Apolipoprotein F, APOF, Apo-F, DKFZp781G18150, MGC22520

Gene ID: 319

NCBI Accession: NP_001629