APOLD1 antibody (C-Term)

Catalog No. ABIN302176

This Goat Polyclonal antibody specifically detects APOLD1 in ELISA, WB and FACS. It exhibits reactivity toward Human.

Quick Overview for APOLD1 antibody (C-Term) (ABIN302176)

Target: APOLD1 (Apolipoprotein L Domain Containing 1 (APOLD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOLD1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-APOLD1 Antibody

Binding Specificity: C-Term

Purpose: APOLD1 / VERGE

Sequence: KSSRGHDLKI SADQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KSSRGHDLKISADQ, from the C Terminus of the protein sequence according to NP_110444.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 35 kDa band observed in lysates of cell lines A431, Jurkat and U251 (calculated MW of 30.5 kDa according to Human NP_001123887.1).This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APOLD1

Target: APOLD1 (Apolipoprotein L Domain Containing 1 (APOLD1))

Alternative Name: APOLD1

Background: APOLD1, apolipoprotein L domain containing 1, DKFZP434F0318, FLJ25138, VERGE

Gene ID: 81575

NCBI Accession: NP_110444