CDw60 antibody

Catalog No. ABIN3023775

The Mouse Monoclonal anti-CDw60 antibody has been validated for IHC (fro), FACS and IF. It is suitable to detect CDw60 in samples from Human.

Quick Overview for CDw60 antibody (ABIN3023775)

Target: CDw60

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: UM4D4

Product Details

Characteristics: This mAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.

Purification: PEG precipitation

Immunogen: The human rheumatoid synovial T cell line (ST-1) was used as the immunogen for the CDw60 antibody.

Isotype: IgM

Application Details

Application Notes: Optimal dilution of the CDw60 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CDw60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: CDw60

Background: This mAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.