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CDw60 antibody

Reactivity: Human IHC (fro), FACS, IF Host: Mouse Monoclonal UM4D4 unconjugated
Catalog No. ABIN6251841
  • Target
    CDw60
    Reactivity
    Human
    Host
    • 3
    Mouse
    Clonality
    • 3
    Monoclonal
    Conjugate
    • 3
    Un-conjugated
    Application
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
    Purification
    PEG precipitation
    Immunogen
    The human rheumatoid synovial T cell line (ST-1) was used as the immunogen for the CDw60 antibody.
    Clone
    UM4D4
    Isotype
    IgM
  • Application Notes
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

    Optimal dilution of the CDw60 antibody should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    CDw60
    Background
    This mAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
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