Mitochondria antibody

Catalog No. ABIN3023781

The Mouse Monoclonal anti- antibody has been validated for WB, IF and IHC (p). It is suitable to detect in samples from Human.

Quick Overview for Mitochondria antibody (ABIN3023781)

Target: Mitochondria

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM198

Product Details

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.

Purification: Protein G affinity chromatography

Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Mitochondria

Background: This mAb recognizes a 60  kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.