pan Keratin antibody

Catalog No. ABIN3024003

The Mouse Monoclonal anti-pan Keratin antibody has been validated for IF, FACS, WB and IHC (p). It is suitable to detect pan Keratin in samples from Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit and Chicken.

Quick Overview for pan Keratin antibody (ABIN3024003)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Dog, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PAN-CK (Cocktail)

Product Details anti-pan Keratin Antibody

Purification: Protein G affinity chromatography

Immunogen: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the pan Cytokeratin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the pan Cytokeratin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Pan Cytokeratin

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 55  kDa (CK7), 52  kDa (CK8), 56.5  kDa (CK10), 53  kDa (CK13), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 46  kDa (CK17), 45  kDa (CK18) and 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.