VLDLR antibody (C-Term)

Catalog No. ABIN3024045

The Mouse Monoclonal anti-VLDLR antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect VLDLR in samples from Human and Rat.

Quick Overview for VLDLR antibody (C-Term) (ABIN3024045)

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VLDLR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: VLPR-1

Product Details anti-VLDLR Antibody

Binding Specificity: C-Term

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein from the C-terminal region was used as the immunogen for this VLDLR antibody.

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the VLDLR antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue is enhanced by boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9 for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the VLDLR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for VLDLR

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Alternative Name: VLDLR

Background: Very Low Density Lipoprotein Receptor (VLDLR/VLDL receptor) is a member of the LDL receptor gene family which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDL receptor is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.

Gene ID: 7436

Pathways: Cellular Response to Molecule of Bacterial Origin