ADIPOQ antibody

Catalog No. ABIN3024137

This Mouse Monoclonal antibody specifically detects ADIPOQ in ELISA, FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ADIPOQ antibody (ABIN3024137)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADIPOQ antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ADPQ-1

Product Details anti-ADIPOQ Antibody

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this Adiponectin antibody.

Isotype: IgG2b

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Adiponectin antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,ELISA: order BSA free format for coating,IHC (FFPE): 1-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Adiponectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Background: Adiponectin (commonly called ADIPO, or by the gene name ADIPOQ) is an adipocytokine, a hormone produced in adipose tissue. It is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma ADIPO levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women, levels tend to be higher than in men, which may be due to androgens suppressing ADIPO levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low ADIPO levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

Gene ID: 9370