Melanoma antibody
Quick Overview for Melanoma antibody (ABIN3024167)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Characteristics
- Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
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Purity
- Protein G affinity chromatography
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Immunogen
- Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
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Isotype
- IgG1
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anti-Melanoma antibody
Reactivity: Human, Horse IHC (p), IF, FACS Host: Mouse Monoclonal NKI-beteb unconjugated
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Application Notes
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Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
Restrictions
- For Research Use only
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Buffer
- 1 mg/mL in sterile PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- -20 °C,4 °C
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Storage Comment
- Store the Melanoma antibody PNL2 at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Melanoma
Target
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