Recombinant CAD antibody

Catalog No. ABIN3024259

This anti-CAD antibody is a Rabbit Monoclonal antibody detecting CAD in IHC (p) and IF. Suitable for Human.

Quick Overview for Recombinant CAD antibody (ABIN3024259)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CAD antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: RMCDN1-1

Product Details anti-CAD Antibody

Purification: Protein A affinity chromatography

Immunogen: A recombinant full length human protein was used as the immunogen for the CAD antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the CAD antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CAD antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: Caldesmon is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). [UniProt]

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process