CD8 antibody (C-Term, Cytoplasmic Domain)

Catalog No. ABIN3024487

This anti-CD8 antibody is a Mouse Monoclonal antibody detecting CD8 in FACS, IF and IHC (p). Suitable for Human.

Quick Overview for CD8 antibody (C-Term, Cytoplasmic Domain) (ABIN3024487)

Target: CD8

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C8-144B

Product Details anti-CD8 Antibody

Binding Specificity: C-Term, Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogen: A 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain was used as immunogen for this CD8 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistology (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2),

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: CD8 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.