Chromogranin A antibody

Catalog No. ABIN3024551

This Mouse Monoclonal antibody specifically detects Chromogranin A in IF, FACS and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Monkey and Pig.

Quick Overview for Chromogranin A antibody (ABIN3024551)

Target: Chromogranin A (CHGA)

Reactivity: Human, Rat, Mouse, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LK2H10

Product Details anti-Chromogranin A Antibody

No Cross-Reactivity: Guinea Pig, Sheep (Ovine), Rabbit

Purification: Protein G purified monoclonal antibody

Immunogen: Human pheochromocytoma cells were used as the immunogen for this Chromogranin A antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Chromogranin A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A

Background: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong chromogranin A antibody staining and absence of keratin antibody staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Gene ID: 1113

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling