Fibronectin antibody

Catalog No. ABIN3024767

Product Details anti-Fibronectin Antibody

Target: Fibronectin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Epitope of this mAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Purification: Protein G affinity chromatography

Immunogen: High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody.

Clone: 568

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Fibronectin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30-60 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Fibronectin

Target: Fibronectin

Abstract: Fibronectin Products

Background: Epitope of this mAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440  kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.