Fascin antibody

Catalog No. ABIN3024845

This Mouse Monoclonal antibody specifically detects Fascin in FACS, IF and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for Fascin antibody (ABIN3024845)

Target: Fascin (FSCN1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FSCN1-418

Product Details anti-Fascin Antibody

Purification: Protein G affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the Fascin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: Fascin

Background: Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. [Wiki]