Fascin antibody

Catalog No. ABIN6940606

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Specificity: Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Purification: Purified by Protein A/G

Immunogen: Full length recombinant human FSCN1 protein

Clone: FSCN1-416

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: K562, HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin's lymphoma, Ovarian or Testicular Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1 (FSCN1 Products)

Molecular Weight: 55kDa

Gene ID: 6624

UniProt: Q16658