Involucrin antibody (AA 421-568)

Catalog No. ABIN3024971

This Mouse Monoclonal antibody specifically detects Involucrin in IF, FACS and IHC (p). It exhibits reactivity toward Human, Dog, Pig, Gorilla and Owl Monkey.

Quick Overview for Involucrin antibody (AA 421-568) (ABIN3024971)

Target: Involucrin (IVL)

Reactivity: Human, Dog, Pig, Gorilla, Owl Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM259

Product Details anti-Involucrin Antibody

Binding Specificity: AA 421-568

No Cross-Reactivity: Mouse (Murine)

Purification: Protein G affinity chromatography

Immunogen: Purified involucrin from human keratinocytes was used as the immunogen for this Involucrin antibody. Its epitope maps between amino acids 421-568 of human involucrin.

Isotype: IgG1 kappa

Application Details

Application Notes: The optimal dilution of the Involucrin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by digestion with Trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37oC. Enzyme digestion is better than citrate buffer based epitope unmasking.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.