Laminin gamma 1 antibody

Catalog No. ABIN3025186

The Rat Monoclonal anti-Laminin gamma 1 antibody (Clone A5) (ABIN3025186) specifically detects Laminin gamma 1 in IHC (p), IF and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Laminin gamma 1 antibody (ABIN3025186)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: A5

Product Details anti-Laminin gamma 1 Antibody

Purification: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Laminin gamma 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: Laminin gamma 1

Background: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210  kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.