FTL antibody (AA 38-165)

Catalog No. ABIN3025210

This anti-FTL antibody is a Mouse Monoclonal antibody detecting FTL in WB, IF, FACS and IHC (p). Suitable for Human.

Quick Overview for FTL antibody (AA 38-165) (ABIN3025210)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FTLC1-1

Product Details anti-FTL Antibody

Binding Specificity: AA 38-165

Purification: Protein G affinity chromatography

Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

Isotype: IgG2b

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Western blot: 1-2 μg/mL,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Ferritin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin (Light Chain Specific)

Background: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Gene ID: 2512

Pathways: Transition Metal Ion Homeostasis