MLANA antibody

Catalog No. ABIN3025225

This anti-MLANA antibody is a Mouse Monoclonal antibody detecting MLANA in IF, FACS and IHC (p). Suitable for Human and Horse.

Quick Overview for MLANA antibody (ABIN3025225)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM342

Product Details anti-MLANA Antibody

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this anti-Melan-A antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: The optimal dilution of the anti-Melan-A antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: Melan-A

Background: Melan-A/MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.