MUC6 antibody

Catalog No. ABIN3025256

This Mouse Monoclonal antibody specifically detects MUC6 in IF, WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for MUC6 antibody (ABIN3025256)

Target: MUC6 (Mucin 6, Oligomeric Mucus/gel-Forming (MUC6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC6 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MUC6-916

Product Details anti-MUC6 Antibody

No Cross-Reactivity: Rat (Rattus)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Mucin-6 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Mucin-6 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Mucin-6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MUC6

Target: MUC6 (Mucin 6, Oligomeric Mucus/gel-Forming (MUC6))

Alternative Name: Mucin-6

Background: MUC6 or gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2, Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.