PDE9A antibody

Catalog No. ABIN302526

The Rabbit Polyclonal anti-PDE9A antibody (ABIN302526) specifically detects PDE9A in IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDE9A antibody (ABIN302526)

Target: PDE9A (phosphodiesterase 9A (PDE9A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE9A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDE9A Antibody

Specificity: This antibody detects Phosphodiesterase 9A (PDE9A) at internal domain.

Purification: Immunoaffinity chromatography

Immunogen: PDE9A antibody was raised against synthetic peptide from human PDE9A.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for PDE9A

Target: PDE9A (phosphodiesterase 9A (PDE9A))

Alternative Name: PDE9A

Background: Phosphodiesterase 9A is a cGMP-specific phosphodiesterase. PDE9A activity is inhibited by zaprinast, but it is insensitive to rolipram, vinpocetine, SKF-94120, dipyridamole, and IBMX. More than 20 alternatively spliced variants have been reported (Rentero et al. 2003). PDE9A has been implicated in penile erectile dysfunction, as well as in functional disturbances in which intraneuronal signal transmission via second messengers are important in the pathophysiology (example, bipolar affective disorder). PDE9A is the only cGMP-specific PDE with significant expression in the forebrain, and as such is likely to function in NO-cGMP signaling.Synonyms: 5'-cyclic phosphodiesterase 9A, HSPDE9A2, High affinity cGMP-specific 3', Phosphodiesterase 9A, Phosphodiesterase PDE9A21

Gene ID: 5152

NCBI Accession: NP_001001567

UniProt: O76083