p40 (p63 Delta) (AA 5-17) antibody

Catalog No. ABIN3025362

The Rabbit Polyclonal anti- antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect in samples from Human, Cow, Mouse and Rat.

Quick Overview for p40 (p63 Delta) (AA 5-17) antibody (ABIN3025362)

Target: p40 (p63 Delta)

Reactivity: Human, Cow, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Binding Specificity: AA 5-17

Purification: Protein A affinity chromatography

Immunogen: Amino acids ENNAQTQFSEPQY (aa 5-17) of human p40 / p63 delta were used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p40 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the p40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: p40 (p63 Delta)

Alternative Name: p40

Background: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p40 antibody's ability to distinguish between the tumor types appears to be superior when compared to p63. The ability to utilize a p40 antibody probe as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Gene ID: 8626