PLAP antibody

Catalog No. ABIN3025400

This Mouse Monoclonal antibody specifically detects PLAP in FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PLAP antibody (ABIN3025400)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ALPP-516

Product Details anti-PLAP Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: Placental Alkaline Phosphatase

Background: PLAP is a tissue specific, trophoblast-derived, 70  kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.