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PODXL antibody (Extracellular Domain, Internal Region)

This Mouse Monoclonal antibody specifically detects PODXL in FACS, IF and IHC (p). It exhibits reactivity toward Human, Rat and Rabbit.
Catalog No. ABIN3025409

Quick Overview for PODXL antibody (Extracellular Domain, Internal Region) (ABIN3025409)

Target

See all PODXL Antibodies
PODXL (Podocalyxin-Like (PODXL))

Reactivity

  • 106
  • 27
  • 14
  • 11
  • 1
  • 1
Human, Rat, Rabbit

Host

  • 68
  • 55
  • 1
Mouse

Clonality

  • 70
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Monoclonal

Conjugate

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This PODXL antibody is un-conjugated

Application

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

2A4
  • Binding Specificity

    • 23
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    • 9
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    Extracellular Domain, Internal Region

    Purification

    PEG precipitation

    Immunogen

    A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

    Isotype

    IgM
  • Application Notes

    Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Preservative

    Azide free

    Storage

    4 °C,-20 °C

    Storage Comment

    Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target

    PODXL (Podocalyxin-Like (PODXL))

    Alternative Name

    Podocalyxin

    Background

    Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

    Pathways

    Tube Formation
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