PODXL antibody (Extracellular Domain, Internal Region)
Quick Overview for PODXL antibody (Extracellular Domain, Internal Region) (ABIN3025409)
Target
See all PODXL AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- Extracellular Domain, Internal Region
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Purification
- PEG precipitation
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Immunogen
- A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.
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Isotype
- IgM
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Application Notes
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Optimal dilution of the Podocalyxin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- PODXL (Podocalyxin-Like (PODXL))
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Alternative Name
- Podocalyxin
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Background
- Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
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Pathways
- Tube Formation
Target
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