ACTH antibody (AA 1-24)

Catalog No. ABIN3025414

The Mouse Monoclonal anti-ACTH antibody (Clone AH26) (ABIN3025414) specifically detects ACTH in IF, FACS and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ACTH antibody (AA 1-24) (ABIN3025414)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AH26

Product Details anti-ACTH Antibody

Binding Specificity: AA 1-24

Purification: Protein G affinity chromatography

Immunogen: A synthetic peptide corresponding to aa 1-24 of human ACTH was used as the immunogen for this ACTH antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH / Synacthen

Target Type: Hormone

Background: POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH (also called Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. Clone AH26 antibody is specific to Synacthen (aa 1-24 of ACTH) and does not react with CLIP (aa 17-39). ACTH is produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Specific antibody is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) that cause paraneoplastic syndromes by secreting ACTH.

Gene ID: 5443

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity