PTH antibody

Catalog No. ABIN3025443

The Mouse Monoclonal anti-PTH antibody has been validated for IF, FACS and IHC (p). It is suitable to detect PTH in samples from Human.

Quick Overview for PTH antibody (ABIN3025443)

Target: PTH (Parathyroid Hormone (PTH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTH antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3H9-PTH-1175

Product Details anti-PTH Antibody

Purification: Protein G affinity chromatography

Immunogen: Synthetic peptides corresponding to amino acids 1 to 34 (clone 3H9) and 32-115 (clone PTH/1175) were used as the immunogen for the PTH antibody cocktail.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PTH antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PTH antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PTH

Target: PTH (Parathyroid Hormone (PTH))

Alternative Name: PTH

Target Type: Hormone

Background: PTH is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process