ODC1 antibody

Catalog No. ABIN3025563

The Mouse Monoclonal anti-ODC1 antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect ODC1 in samples from Human and Rat.

Quick Overview for ODC1 antibody (ABIN3025563)

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ODC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ODC1-487

Product Details anti-ODC1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the Ornithine Decarboxylase antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the Ornithine Decarboxylase antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Ornithine Decarboxylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Alternative Name: Ornithine Decarboxylase

Background: Recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.