ST6GAL1 antibody

Catalog No. ABIN3025661

This Mouse Monoclonal antibody specifically detects ST6GAL1 in WB, FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ST6GAL1 antibody (ABIN3025661)

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ST6GAL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LN1

Product Details anti-ST6GAL1 Antibody

Purification: PEG precipitation

Immunogen: Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes were used as the immunogen for the CDw75 antibody.

Isotype: IgM

Application Details

Application Notes: Optimal dilution of the CDw75 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CDw75 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ST6GAL1

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Alternative Name: CDw75

Background: Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This mAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.