NUMA1 antibody

Catalog No. ABIN3025683

Product Details anti-NUMA1 Antibody

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: PEG precipitation

Immunogen: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Clone: A73-B-D12

Isotype: IgM kappa

Application Details

Application Notes: Optimal dilution of the NuMA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA (NUMA1 Products)

Background: Recognizes a phosphorylated protein of 228  kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200  kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase