CAD antibody

Catalog No. ABIN3025776

The Mouse Monoclonal anti-CAD antibody is suitable to detect CAD in samples from Human. It has been validated for IHC (p), IF and FACS.

Quick Overview for CAD antibody (ABIN3025776)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAD antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CALD1-820-h-CALD

Product Details anti-CAD Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein (CALD1/820) and crude human uterus extract (h-CALD) were used as the immunogen for the CAD antibody cocktail.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CAD antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CAD antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: Recognizes a protein of 150  kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150  kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80  kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This mAb recognizes only the 150  kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process